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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and so could add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital part in promoting cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants were being reported but in depth nucleotide sequence facts is not really yet accessible.

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Blocking peptides are peptides that bind specifically into the focus on antibody and block antibody binding. These peptide generally contains the epitope recognized by the antibody.

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This manual outlines further more feasible optimization of mobile and immunoassay parameters to ensure the absolute best effects are...

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This information outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best effects are...

Antibodies certain to the blocking peptide not bind to the epitope over the focus on protein. By evaluating the staining through the blocked antibody versus the antibody by yourself, one can see which staining is unique.

Goal Information Cyclin E belongs to your hugely conserved cyclin family members, whose users are characterized by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show distinctive expression and degradation patterns click here which contribute to your temporal coordination of each and every mitotic function. Cyclin E varieties a posh with and functions like a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates in the G1-S stage boundary which is degraded as cells development via S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...

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